Enzyme lectin assay.

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Lectin and enzyme relationships in microbiology

Examples of inter- and intramolecular direct co-functioning of lectins and enzymes are summarized. The data indicate importance of lectin-enzyme relationships in molecular and clinical microbiology and medical biotechnology. Lectin-enzyme relationships have extended prospects in medicine and biotechnology.

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Probing multivalent carbohydrate-lectin interactions by an enzyme-linked lectin assay employing covalently immobilized carbohydrates.

We report here the synthesis of a series of mono- to trivalent N-acetylglucosamine (GlcNAc) derivatives as ligands for the plant lectin wheat germ agglutinin (WGA). Their WGA binding potencies were determined by an established enzyme-linked lectin assay (ELLA) employing microtiter plates with non-covalently immobilized porcine stomach mucin (PSM) as reference ligand and an ELLA with a new GlcNA...

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lectin and enzyme relationships in microbiology

examples of inter- and intramolecular direct co-functioning of lectins and enzymes are summarized. the data indicate importance of lectin-enzyme relationships in molecular and clinical microbiology and medical biotechnology. lectin-enzyme relationships have extended prospects in medicine and biotechnology.

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An Innovative Pseudotypes-Based Enzyme-Linked Lectin Assay for the Measurement of Functional Anti-Neuraminidase Antibodies

Antibodies (Ab) to neuraminidase (NA) play a role in limiting influenza infection and might help reduce the disease impact. The most widely used serological assay to measure functional anti-NA immune responses is the Enzyme-Linked Lectin Assay (ELLA) which relies on hemagglutinin (HA) mismatched virus reassortants, or detergent treated viruses as the NA source to overcome interference associate...

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Optimization of the enzyme-linked lectin assay for enhanced glycoprotein and glycoconjugate analysis.

Lectins are proteins capable of recognizing and binding to specific oligosaccharide structures found on glycoproteins and other biomolecules. As such, they have utility for glycoanalytical applications. One common difficulty encountered in the application of these proteins, particularly in multiwell plate assay formats known as enzyme-linked lectin assays (ELLAs), is finding appropriate blockin...

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ژورنال

عنوان ژورنال: SEIBUTSU BUTSURI KAGAKU

سال: 1987

ISSN: 0031-9082,1349-9785

DOI: 10.2198/sbk.31.165